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KMID : 0613820100200081221
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Journal of Life Science
2010 Volume.20 No. 8 p.1221 ~ p.1229
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Anti-Inflammmatiry Effects of Nerium indicum Ethanol Extracts through Suppression of NF-kappaB Activation
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Ko Seog-Soon
Park Cheol
Park Sang-Eun
Hong Sang-Hoon
Kim Byung-Woo
Choi Yung-Hyun
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Abstract
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Nerium indicum, an India-Pakistan-originated shrub belonging to the oleander family, is reported to possess many pharmacological activities including cardiac muscle stimulation, and anti-diabetes, anti-angiogenesis, anti-cancer and neuro-protective activities. However, the anti-inflammatory properties of N. indicum were unclear. In this study, we investigated the effects of ethanol extract of the N. indicum leaf and stem (ENIL and ENIS) on the expression of anti-inflammatory mediators in U937 human pre-monocytic cell models. In U937 cells stimulated with phorbol 12-myristate-13-acetate (PMA), pre-treatment with ENIS significantly inhibited the expression of both cyclooxygenase-2 (COX-2) mRNA and protein, which are associated with inhibition of the release of prostaglandin E₂ (PGE₂), whereas the inhibitory effects appeared weakly in ENIL. Moreover, ENIS significantly attenuated PMA-induced IkappaB (IκB) degradation and suppressed elevated nuclear factor kappa B (NF-κB) nuclear translocation. Taken together, these findings provide important new insights that N. indicum exhibits anti-inflammatory properties by suppressing the transcription of pro-inflammatory cytokine genes through the NF-kB signaling pathway.
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KEYWORD
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Nerium indicum, cyclooxygenase-2, prostaglandin E₂, nuclear factor-kappaB
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